Fig. 6.

The Ndc80 internal loop binds to Dis1 and Alp7–Alp14, thereby recruiting the Klp5–Klp6 complex to control mitotic progression. A schematic view of mitotic progression from prometaphase to anaphase A. In prophase, Dis1 localises to the Ndc80 internal loop. Microtubules nucleate, assemble and elongate from the SPB (not shown) towards the kinetochores. The Alp7–Alp14 complex is delivered by the microtubules. In metaphase, this complex reaches the Ndc80 internal loop, thereby providing a robust kinetochore-microtubule attachment. Alp7 loads the Klp5–Klp6–PP1 complex to the attached kinetochore. Recruitment of the Klp5–Klp6–PP1 complex facilitates silencing of the spindle assembly checkpoint, thereby inducing timely anaphase onset. The Klp5–Klp6 complex then couples chromosome movement to depolymerising microtubule plus-ends to ensure efficient anaphase chromosome movement.