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. Author manuscript; available in PMC: 2015 Sep 12.
Published in final edited form as: Neuroscience. 2014 Feb 4;276:72–86. doi: 10.1016/j.neuroscience.2014.01.051

Figure 5. Loss of c-Myc leads to decreased histone acetylation at its target gene promoters.

Figure 5

Chromatin was isolated from mouse OPCs 72h post-infection with either scramble shRNA c-Myc or scrambled control, and immunoprecipitated with antibodies specific for acetylated lysine 9 and lysine 14 on histone 3. After reverse cross-linking, the bound DNA was amplified using primer sets specific for the promoters of the c-Myc target genes (a) or for an independent gene, such as Mbp (b). A mock immunoprecipitation was used as negative control. Experiment was performed in duplicate.