Table 3.
Reduction of sperm DNA fragmentation after treatment (subgroups)
| Group 1 (DGC-SU) | Group 2 (DGC-MACS-SU) | Group 3 (DGC-SU-MACS) | Group 4 (MACS-DGC-SU) | Group 5 (MACS-SU) | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (n) | T0 (%) | T1 (%) | Reduction sDNAfrag (%) | (n) | T0 (%) | T1 (%) | Reduction sDNAfrag (%) | (n) | T0 (%) | T1 (%) | Reduction sDNAfrag (%) | (n) | T0 (%) | T1 (%) | Reduction sDNAfrag (%) | (n) | T0 (%) | T1 (%) | Reduction sDNAfrag (%) | |
| Asthenozoospermia | 9 | 4.0 ± 1.6 | 0.7 ± 0.6 | 82.9 ± 12.0a | 9 | 3.8 ± 2.3 | 0.7 ± 0.6 | 78.3 ± 21.4a | 16 | 7.4 ± 6.9 | 3.6 ± 4.3 | 56.7 ± 25.0b | 8 | 7.2 ± 5.9 | 1.4 ± 1.6 | 83.0 ± 11.0a | 13 | 4.9 ± 3.0 | 1.5 ± 1.9 | 67.2 ± 28.0a,b |
| control | 11 | 4.6 ± 3.7 | 1.4 ± 1.5 | 65.5 ± 29.3 | 11 | 6.0 ± 4.4 | 1.3 ± 1.6 | 79.4 ± 20.5 | 4 | 11.3 ± 16.7 | 1.5 ± 1.5 | 42.2 ± 18.4 | 12 | 4.4 ± 3.0 | 0.8 ± 1.0 | 83.5 ± 18.2 | 7 | 3.2 ± 1.6 | 0.5 ± 0.4 | 85.3 ± 13.3 |
| Teratozoospermia | 9 | 5.3 ± 3.9 | 1.5 ± 1.5 | 72.0 ± 22.4 | 10 | 5.4 ± 2.3 | 1.4 ± 1.7 | 79.3 ± 21.3 | 8 | 11.4 ± 11.3 | 3.8 ± 4.2 | 45.4 ± 26.6 | 8 | 5.8 ± 4.4 | 1.4 ± 1.2 | 76.8 ± 17.3 | 10 | 5.7 ± 2.9 | 1.9 ± 2.0 | 66.0 ± 26.5 |
| control | 11 | 3.4 ± 1.3 | 0.8 ± 1.0 | 74.5 ± 26.7 | 10 | 4.7 ± 4.8 | 0.7 ± 0.5 | 78.6 ± 20.5 | 12 | 6.1 ± 7.2 | 2.7 ± 3.9 | 59.5 ± 21.6 | 12 | 5.3 ± 4.7 | 0.8 ± 1.3 | 87.6 ± 12.9 | 10 | 2.9 ± 1.7 | 0.5 ± 0.4 | 81.1 ± 22.4 |
| Asthenoteratozoospermia | 4 | 3.7 ± 2.2 | 1.0 ± 0.8 | 78.1 ± 16.9 | 6 | 4.6 ± 2.4 | 0.7 ± 0.8 | 87.5 ± 10.2 | 5 | 6.6 ± 6.0 | 2.2 ± 1.3 | 56.7 ± 24.6 | 5 | 5.4 ± 4.5 | 1.0 ± 1.0 | 81.6 ± 6.4 | 9 | 5.9 ± 3.0 | 2.0 ± 2.1 | 64.5 ± 27.7 |
| control | 16 | 4.4 ± 3.1 | 1.1 ± 1.3 | 72.2 ± 26.1 | 14 | 5.2 ± 4.2 | 1.2 ± 1.4 | 75.2 ± 22.7 | 15 | 8.7 ± 10.1 | 3.5 ± 4.5 | 52.9 ± 24.7 | 15 | 5.6 ± 4.6 | 1.1 ± 1.4 | 83.9 ± 17.5 | 11 | 3.0 ± 1.6 | 0.5 ± 0.4 | 80.9 ± 21.3 |
| Total | 20 | – | – | – | 20 | – | – | – | 20 | – | – | – | 20 | – | – | – | 20 | – | – | – |
Values are expressed as mean ± standard deviation
Different superscript letters within a row indicate statistically significant differences (p < 0.05)
n number of patients studied; sDNAfrag, sperm DNA fragmentation
Asthenozoospermia (<25 % rapid progressive motility; control: ≥25 % rapid progressive motility)
Asthenoteratozoospermia (<25 % rapid progressive motility + <4 % normal morphology; control: ≥25 % rapid progressive motility + ≥4 % normal morphology)
Teratozoospermia (<4 % normal morphology; control: ≥4 % normal morphology)
Controls, samples showing each specific spermiogram parameter normal (≥25 % rapid progressive motility or ≥4 % normal morphology)
T0, raw semen; T1, after treatment