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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Thromb Haemost. 2014 Nov 11;13(1):72–81. doi: 10.1111/jth.12755

Figure 2. Identification of A2 and C2 domain epitopes targeted by patient plasmas.

Figure 2

A competition ELISA was performed with anti-A2 and -C2 domain MAbs competing against human inhibitor IgG for binding to hFVIII. HFVIII first was blocked with patient plasma and then incubated with individual biotinylated MAbs. Patient ID is listed along the y-axis in descending order of the Bethesda titers measured for each sample and individual MAbs ID/group are listed across the top. Absence of competition is represented by black shading, and white shading designates data not determined. Competition is defined as a reduction of kinetic signal outside 2 standard deviations of control kinetic rates and is represented as yellow shading.