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Quantitative real-time PCR results demonstrating the relative expression of αSMA mRNA. TGFβ1 treatment (1ng/ml) induced significant αSMA formation (four-fold) in myofibroblasts and 200 μg/ml PFD treatment significantly decreased TGFβ1-induced myofibroblast formation. Error bars indicate standard error.
* denotes P < 0.001 (untreated control versus TGFβ1 treatment)
** denotes P < 0.001 (TGFβ1 treatment versus TGFβ1 + PFD treatment).
