FIGURE 2.
Effect of POPS on GCase membrane association and activity. GCase binding to POPC (circles) and equimolar POPC/POPS (squares) stBLMs is quantified by SPR spectroscopy. One pixel change corresponds to a SPR minimum angle change of 0.0067°. Solid lines, fits to a Langmuir adsorption process (equilibrium dissociation constants (Kd) are 210 ± 6 nm and 6.8 ± 1.7 μm for POPC/POPS and POPC, respectively). Error bars, SPR signal variations at the plateau of each addition. Inset, normalized GCase activity measured in vesicles containing different amounts of POPS (0–50%). Error bars, S.D. values (n = 2).