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Experimental design and test results of lipid and GLA produced by Cunninghamella bainieri 2A1 in the submerged bioreactor.
| Run | X 1 | X 2 | Lipid concentration (g/L) | GLA concentration (mg/L) |
|---|---|---|---|---|
| 1 | −1 | 1 | 4.23 | 567.5 |
| 2 | 0 | 0 | 4.53 | 573.12 |
| 3 | 0 | −1.41 | 1.23 | 144.0 |
| 4 | 0 | 0 | 4.60 | 602.82 |
| 5 | 1 | −1 | 2.35 | 322.65 |
| 6 | 0 | 0 | 4.18 | 539.09 |
| 7 | 1.41 | 0 | 4.74 | 747.72 |
| 8 | 0 | 0 | 4.62 | 591.0 |
| 9 | 1 | 1 | 3.95 | 621.0 |
| 10 | −1.41 | 0 | 3.01 | 433.5 |
| 11 | 0 | 0 | 4.31 | 573.12 |
| 12 | 0 | 1.41 | 4.34 | 567.36 |
| 13 | −1 | −1 | 1.125 | 145.0 |
X 1: aeration rate (vvm); X 2: agitation rate (rpm).
