Fig. 3.

Integrative map of the narrow-leafed lupin genome: BAC-FISH-based assignment of linkage groups to their corresponding chromosomes. a Localization of BAC clones 043C18, 015P08, 138N02, 115C21, 072O21, and 017B07 to L. angustifolius chromosomes. The genetic positions of the clones are represented by the BES-derived genetic markers, 043C18_3F3, 015P08_3, 138N02_3F3, 115C21_5, 072O21_3F2 + 072O21_3F2E, and 017B07_3, respectively. BAC clone DNA was labeled with tetramethylrhodamine-5-dUTP (red signals) or digoxigenin-11-dUPT (green signals). Overlapping clones produced yellow signals. Chromosomes were counterstained with DAPI. Scale bar = 5 μm. b Idiogram of L. angustifolius (12 of 20 chromosomes) showing the available BAC chromosome-specific markers, including our novel markers and the previously reported markers (Książkiewicz et al. 2013; Lesniewska et al. 2011)