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. 2015 Jan 15;5:7794. doi: 10.1038/srep07794

Figure 4. Semi-automatic detection of chloroplasts in a TEM image of Arabidopsis thaliana embryo.

Figure 4

(A) TEM image used to detect chloroplasts (inset). Image resolution: 2000 × 2000 pixels. (B) Results of semi-automatic detection. Green rectangles show ‘true' chloroplasts, which were confirmed by visual inspection; red boxed regions show semi-automatically detected windows. (C, D) Relationship between detection area and true (C) or false (D) positive detection rates with random selection of randomly-located windows (filled circles) and proposed methods (open circles). Data are mean ± SD from three independent experiments. (E) Relationship between true and false positive detection rates with random selection of randomly-located windows (filled circles) and proposed methods (open circles). Data are mean ± SD from three independent experiments. (F) Comparison of true positive rates. Mean number of false positive windows was 33.0 (random detection) (N = 4) and 6.67 (proposed method) (N = 6). Data are mean ± SD. Significance was determined using Mann–Whitney's U-test (p-value, **<0.0001).