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. Author manuscript; available in PMC: 2015 Jan 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2014 Jul 14;279(3):253–265. doi: 10.1016/j.taap.2014.06.030

Fig. 1 — Sensitizing effect of gingerol in TRAIL-induced apoptosis of U87 glioblastoma cells. (A and B) Cells were treated with DMSO (sham control) or various concentrations (10–100μM) of gingerol for 24 h. (C, D, and E) Cells were incubated in the presence or absence of TRAIL (50 ng/ml) and/or gingerol (25 μM) for 24 h. (F) Microscopic cell morphologies. Scale bar: 100 μm. (G) The cells were stained with annexin V and propidium iodide (PI), followed by FACS analysis. (H) The cell lysates were analyzed by western blotting using indicated antibodies. Data is presented as arbitrary values and results are expressed as the mean ± SEM. The effects of nicotine were determined using a Student's unpaired t-test. Asterisk * represents statistically significantly difference between control and gingerol-treated cells at p < 0.05.

Fig. 1 — Sensitizing effect of gingerol in TRAIL-induced apoptosis of U87 glioblastoma cells. (A and B) Cells were treated with DMSO (sham control) or various concentrations (10–100μM) of gingerol for 24 h. (C, D, and E) Cells were incubated in the presence or absence of TRAIL (50 ng/ml) and/or gingerol (25 μM) for 24 h. (F) Microscopic cell morphologies. Scale bar: 100 μm. (G) The cells were stained with annexin V and propidium iodide (PI), followed by FACS analysis. (H) The cell lysates were analyzed by western blotting using indicated antibodies. Data is presented as arbitrary values and results are expressed as the mean ± SEM. The effects of nicotine were determined using a Student's unpaired t-test. Asterisk * represents statistically significantly difference between control and gingerol-treated cells at p < 0.05.