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. Author manuscript; available in PMC: 2015 Jan 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2014 Jul 14;279(3):253–265. doi: 10.1016/j.taap.2014.06.030

Fig. 2 — Gingerol can regulate the expression of death receptors (DR) 5, but not DR4. (A) U87 cells were treated with indicated gingerol doses (0, 10, 25, 50 μM) for 24 h or 25 μM gingerol at an indicated time (0, 4, 8, 16, 24 h). Western blotting analysis was performed with DR4 and DR5 antibodies. (B) U87 cells were incubated with DMSO or gingerol (25 μM) for 24 h and stained with DR4 and DR5 antibodies, followed by FACS analysis (left panels) and immunocytochemistry (right panels). Scale bar: 100 μm. (C) The indicated cell types were incubated with DMSO or gingerol (25 μM) for 24 h. The cell lysates were analyzed by western blotting using DR4 and DR5 antibodies. (D) U87 cells were transfected with Myc-DR5 plasmid or pcDNA (control). After treatment with TRAIL for 4 h, western blot analysis demonstrated that Myc-DR5-induced DR5 overexpression increased apoptosis compared with pcDNA. (E) U87 cells were incubated with indicated gingerol doses (0, 10, 25, 50 μM) for 24 h or 25 μM gingerol at an indicated time (0, 4, 8, 16, 24 h) and then analyzed by western blotting using p53 antibody. (F) HCT116 (p53+/+) and HCT116 (p53−/−) cells were incubated with indicated gingerol (0, 10, 25, 50 μM) for 24 h. The cell lysates were analyzed by western blotting using p53, DR4, and DR5 antibodies. Data is presented as arbitrary values and results are expressed as the mean ± SEM. The effects of nicotine were determined using a Student's unpaired t-test. *p < 0.05 vs control. (G) Cell viability was determined using the trypan blue dye exclusion assay. Error bars represent the mean ± SE from six separate experiments. *Significant difference between TRAIL and TRAIL + gingerol-treated cells at p < 0.05. These results are representative of data obtained from at least five independent experiments.

Fig. 2 — Gingerol can regulate the expression of death receptors (DR) 5, but not DR4. (A) U87 cells were treated with indicated gingerol doses (0, 10, 25, 50 μM) for 24 h or 25 μM gingerol at an indicated time (0, 4, 8, 16, 24 h). Western blotting analysis was performed with DR4 and DR5 antibodies. (B) U87 cells were incubated with DMSO or gingerol (25 μM) for 24 h and stained with DR4 and DR5 antibodies, followed by FACS analysis (left panels) and immunocytochemistry (right panels). Scale bar: 100 μm. (C) The indicated cell types were incubated with DMSO or gingerol (25 μM) for 24 h. The cell lysates were analyzed by western blotting using DR4 and DR5 antibodies. (D) U87 cells were transfected with Myc-DR5 plasmid or pcDNA (control). After treatment with TRAIL for 4 h, western blot analysis demonstrated that Myc-DR5-induced DR5 overexpression increased apoptosis compared with pcDNA. (E) U87 cells were incubated with indicated gingerol doses (0, 10, 25, 50 μM) for 24 h or 25 μM gingerol at an indicated time (0, 4, 8, 16, 24 h) and then analyzed by western blotting using p53 antibody. (F) HCT116 (p53+/+) and HCT116 (p53−/−) cells were incubated with indicated gingerol (0, 10, 25, 50 μM) for 24 h. The cell lysates were analyzed by western blotting using p53, DR4, and DR5 antibodies. Data is presented as arbitrary values and results are expressed as the mean ± SEM. The effects of nicotine were determined using a Student's unpaired t-test. *p < 0.05 vs control. (G) Cell viability was determined using the trypan blue dye exclusion assay. Error bars represent the mean ± SE from six separate experiments. *Significant difference between TRAIL and TRAIL + gingerol-treated cells at p < 0.05. These results are representative of data obtained from at least five independent experiments.