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. Author manuscript; available in PMC: 2015 Jan 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2014 Jul 14;279(3):253–265. doi: 10.1016/j.taap.2014.06.030

Fig. 4 — Gingerol can regulate the pro-apoptotic signaling of TRAIL through ROS generation. U87 cells were treated with gingerol (25 μM) in the presence or absence of N-acetylcysteine (Lluis et al., 2010) for 24 h, and then incubated with dihydroethidium (DHE) or 2′,7′-dichlorofluorescein (H2DCF), followed by (A) FACS analysis and (B) immunocytochemical analysis. Scale bar: 100 μm. (C) The cells were incubated with indicated drugs (25 μM gingerol and 5 mM NAC) for 24 h, and then western blotting was performed by using the indicated antibodies (Survivin, c-FLIP, BAX, Bcl-2, and Bid). (D) The cells were treated with indicated reagents (TRAIL, gingerol and NAC) for 24 h. The cell lysates were analyzed by western blotting using indicated antibodies (caspase-3,-8 and PARP-1). Data is presented as arbitrary values and results are expressed as the mean ±SEM. The effects of nicotine were determined using a Student's unpaired t-test. *p < 0.05 vs control. These results are representative of data obtained from at least five independent experiments.

Fig. 4 — Gingerol can regulate the pro-apoptotic signaling of TRAIL through ROS generation. U87 cells were treated with gingerol (25 μM) in the presence or absence of N-acetylcysteine (Lluis et al., 2010) for 24 h, and then incubated with dihydroethidium (DHE) or 2′,7′-dichlorofluorescein (H2DCF), followed by (A) FACS analysis and (B) immunocytochemical analysis. Scale bar: 100 μm. (C) The cells were incubated with indicated drugs (25 μM gingerol and 5 mM NAC) for 24 h, and then western blotting was performed by using the indicated antibodies (Survivin, c-FLIP, BAX, Bcl-2, and Bid). (D) The cells were treated with indicated reagents (TRAIL, gingerol and NAC) for 24 h. The cell lysates were analyzed by western blotting using indicated antibodies (caspase-3,-8 and PARP-1). Data is presented as arbitrary values and results are expressed as the mean ±SEM. The effects of nicotine were determined using a Student's unpaired t-test. *p < 0.05 vs control. These results are representative of data obtained from at least five independent experiments.