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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2014 Jul 14;279(3):253–265. doi: 10.1016/j.taap.2014.06.030

Fig. 5.

Fig. 5

Effect of Bcl-2 in the sensitizing function of gingerol. (A) Vector or Bcl-2-overexpressing U87 stable cell lines (upper panel) were treated with TRAIL and gingerol for 24 h. The cell lysates were analyzed by western blotting of Flag antibody. Actin was used to confirm the equal amount of proteins loaded in each lane. All results are representative of the data obtained by five independent experiments. The cells were then analyzed by MTT assay. (B) Bcl-2 was silenced by si-RNA in U87 cells (upper panel). The cells were then treated with TRAIL for 24 h, followed by MTT analysis. Results shown are representative of six independent experiments.