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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2014 Jul 14;279(3):253–265. doi: 10.1016/j.taap.2014.06.030

Fig. 6.

Fig. 6

Astrocytes are resistant to the combined treatment with gingerol and TRAIL. (A) Human astrocytes were treated with or without gingerol for 30 min and further treated with TRAIL for 16 h at the indicated concentrations. Cellular viability was assessed using trypan blue exclusion assay. (B) Following treatment of astrocytes or U87MG cells with 25 μM gingerol for the indicated times, western blotting of DR5 and Bcl-2 antibodies. Actin was used to confirm the equal amount of proteins loaded in each lane. (C) Astrocytes or U87MG cells were treated with 25 μM gingerol alone, 100 ng/ml TRAIL alone or a combination with gingerol and TRAIL for 16 h. Caspase-3/7 activity is shown. Data is presented as arbitrary values and results are expressed as the mean ± SEM. The effects of nicotine were determined using a Student's unpaired t-test. *p < 0.05 vs control. Results shown are representative of six independent experiments.