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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: J Cancer Res Clin Oncol. 2012 Nov 16;139(4):563–572. doi: 10.1007/s00432-012-1352-6

Fig. 6.

Fig. 6

Curcumin inhibits TGF-β/Smad signaling in H358 and A549 cells. Western blot analyses were performed using lysates from ACC-LC-176 (a), H358 (b), and A549 (c) cells treated with TGF-β (5 ng/ ml) and 10 or 20 μM curcumin for 22 h. Luciferase reporter assay. ACC-LC-176 (d), H358 (e), and A549 (f) cells were transiently transfected with p3TP-Lux, (GAGA)9 MLP-Luc, and CMV-βgal reporter plasmids, and then pretreated with curcumin of indicated concentrations for 2 h, and 5 ng/ml TGF-β was added thereafter for 22 h. Cell lysates were used to measure luciferase and β-gal activities, and normalized luciferase activities were presented