Table 2.

Expression of different endothelial markers by the three cell lines isolated in the study

	Cell lines
	PTEC1	PTEC2	PTEC3	AVERAGE
Isotype	5.32 ± 0.57	6.34 ± 0.77	5.69 ± 0.98	5.783 ± 0.83
CD146	6.598 ± 1.08	12.203 ± 2.87	14.727 ± 9.89	11.176 ± 4.93
CD105	91.614 ± 28.56	70.547 ± 17.45	65.707 ± 26.94	75.956 ± 19.62
CD31	27.942 ± 3.75	30.723 ± 4.46	24.54 ± 7.92	28.068 ± 5.89
TIE-2	23.555 ± 2.46	19.973 ± 4.77	29.13 ± 4.04	24.219 ± 4.84
VEGFR1	19.954 ± 5.35	8.68 ± 4.67	12.17 ± 2.78	13.575 ± 5.94
VEGFR2	20.782 ± 3.07	18.545 ± 3.71	25.83 ± 17.55	21.719 ± 8.32
VEGFR3	5.057 ± 2.69	7.811 ± 2.44	10.603 ± 3.92	7.823 ± 3.16

Levels of endothelial markers was detected by cytofluorimetric analysis after cell staining with a specific conjugated Abs. An irrelevant isotypic Ab (isotype) was used as control of aspecific binding. Data represent the median fluorescent intensity (MFI ± SD) detected on the cell isolates at all culture passages used (1–6). All markers were significantly different versus isotypic control (p < 0.001), as evaluated using the Kolmogorov-Smirnov statistical analysis.