Table 1. Primers for Amplification of TEM and SHV Genesa.
Primers | Temperature, °C | Nucleotide Sequences, 5’-3’ | References | Size, bp |
---|---|---|---|---|
SHV | 60 | (2) | 293 | |
F | CGCCTGTGTATTATCTCCCT | |||
R | CGAGTAGTCCACCAGATCCT | |||
TEM | 60 | (2) | 403 | |
F | TTTCGTGTCGCCCTTATTCC | |||
R | ATCGTTGTCAGAAGTAAGTTGG |
aAbbreviations: SHV, Sulfhydryl Variable; F, Forward; TEM, Temoneira; R, Reverse.