Table 2. Thermocycler Program for Amplification of Gene AphA6/aadB/aadA1/aacC1.

NO.	Parameter Steps	Temperature, ◦C	Time
1	initial denaturation	95	3'
2	denaturation	94	1'
3	annealing	55/55/52/52	1'
4	cycle number	35
5	extension	72	45"
6	final extension	72	5'