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. 2014 Dec 8;14:474. doi: 10.1186/1472-6882-14-474

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© Zhang et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of STA on cell surface area and protein/DNA ratio changes induced by NE. (A) Representative fluorescent micrographs of cardiomyocytes; F-actin is visualized with Alexa Fluor 488-conjugated phalloidin stain (green), whereas the nucleus is visualized by DAPI staining (blue), ×400. (B) Quantification of cell surface area, n = 150. (C) Ratio of Protein/DNA, n = 5. (D) Cell viability, n = 4. The data are expressed as the mean ± SEM. *P < 0.05 versus control, ^#P < 0.05 versus NE. NE: norepinephrine, CAR: carvedilol, STA: stachydrine.