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. 2014 Dec 7;14:922. doi: 10.1186/1471-2407-14-922

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© Rondón-Lagos et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of Chr17 using G-Banding, dual-color FISH ( HER2 /CEP17 , STARD3 /CEP17 and TOP2A /CEP17) and M-FISH in the MCF7, T47D, ZR-75-1 and MDA-MB231 not HER2 amplified breast cancer cell lines. Rearranged chromosomes containing a portion of Chr17 are visualized by G-Banding technique on the left and by M-FISH on the right. For M-FISH the classified color of Chr17 is shown in pink, the translocation partners are numbered on the right hand side of the chromosomes and the frequency at which each abnormality was observed is indicated in brackets at the end of each abnormality. CEP17, HER2, STARD3 and TOP2A are shown in the middle by dual-color FISH (HER2/CEP17, STARD3/CEP17, TOP2A/CEP17, respectively) whenever mapped to the corresponding derivatives (CEP17 is green-labeled; HER2, STARD3 and TOP2A genes are red-labeled).