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. 2014 Dec 9;35(1):101–110. doi: 10.1128/MCB.00636-14

FIG 4.

FIG 4

MondoA and mTOR interact. (A) Lysate from HEK293T cells expressing myc-mTOR and MondoA-V5 was fractionated using a Superose 6 10/300 GL column as described in Materials and Methods. A total of 40 μl from the indicated fractions was subjected to SDS-PAGE, and expression of myc-mTOR, MondoA-V5, and raptor was determined by Western blotting. (B) Representative images showing MondoA-V5 (red) and myc-mTOR (green) subcellular localization in HA1ER cells. (C and D) Levels of indicated proteins in anti-myc or anti-V5 immunoprecipitates and lysate prepared from HEK293T cells transfected with the indicated plasmids were determined by Western blotting. (E) Levels of the indicated proteins in anti-IgG or anti-mTOR immunoprecipitates and lysate prepared from HEK293T cells with the indicated detergents was determined by Western blotting. Digi; digitonin. (F) Levels of the indicated proteins in mTOR immunoprecipitates and lysate prepared from wild-type and MondoA-KO MEF cells were determined by Western blotting (*, nonspecific band). (G) Levels of the indicated proteins in anti-myc immunoprecipitates and lysate prepared from HEK293T cells transfected with indicated plasmids were determined by Western blotting.