FIG 4.

Synthetic lethality of Δfub1 Δpba4. (A) Dissection of diploid cells bearing Δfub1 and Δpba4 alleles. Spores in the dashed circles were expected to have Δfub1 and Δpba4 mutations. (B) Comparison of growth levels between single and double mutant strains lacking Fub1 and proteasome-related proteins. Double mutants lacking Fub1 and proteasome-related proteins were obtained by crossing Δfub1 cells with various proteasome-related disruptant cells, followed by tetrad analysis. The resultant strains were streaked onto YPD plates and incubated for 2 days at 27°C or 35°C.