FIG 8.

N-terminal deletion of α7, but not that of α3, suppresses Δfub1 Δpba4 lethality. (A) Dissection of diploid cells bearing Δfub1::hph, Δpba4::kan, and α3ΔN-URA3. In α3ΔN, 9 residues (GSRRYDSRT) were deleted from the N terminus of α3. Dashed circles represent the locations of the spores expected to have Δfub1 Δpba3 α3ΔN triple mutations. Either one of the two spores located in dashed triangles is also expected to have Δfub1 Δpba3 α3ΔN triple mutations. (B) Dissection of diploid cells bearing Δfub1::hph, Δpba4::kan, α3ΔN-URA3, and α7ΔN-HIS3. In α7ΔN, 11 residues (TSIGTGYDLSN) were deleted from the N terminus of α7. The circled viable colonies have α7ΔN mutations (His⁺) along with Δfub1 Δpba3 but do not have α3ΔN (Ura⁻). (C) Inability of α7ΔN to suppress Δpba4 phenotypes. Wild-type (WT), Δpba4, α7ΔN, and α7ΔNΔpba4 cells were streaked onto a plate containing YPD plus 5 mM AZC and incubated for 2 days at 27°C (left). The same cells were also streaked onto a YPD plate and incubated for 1 day at 37°C (right).