TABLE 2.
List of plasmids used in this study
| Plasmid | Description | Source (reference)a |
|---|---|---|
| D4 | pRS316, low-copy-number URA3 vector | Thomas E. Dever |
| D8 | pRS426, 2μ URA3 vector | Thomas E. Dever |
| D63 | HAC1-WT in D4-KpnI/BamHI sites | This study |
| D712 | HAC1-GG1143-1144CC in low-copy-number LEU2 vector | This study |
| D713 | HAC1-GG1143-1144CC in low-copy-number URA3 vector | This study |
| D759 | HAC1-CGC1145-1147GCG in low-copy-number URA3 vector | This study |
| D760 | HAC1-GCG1180-1182CGC in low-copy-number URA3 vector | This study |
| D761 | HAC1-AC1183-1184CG in low-copy-number URA3 vector | This study |
| D64 | HAC1-ΔBE (A1156-U1174) in low-copy-number URA3 vector | This study |
| D144 | HAC1-G661C in low-copy-number URA3 vector | This study |
| D69 | HAC1Δi in low-copy-number URA3 vector | This study |
| D848 | HAC1Δi-G661C in low-copy-number URA3 vector | This study |
| D559 | HAC1Δi-GG1143-1144CC in low-copy-number URA3 vector | This study |
| D72 | IRE1 WT in low-copy-number LEU2 vector | Lee et al. (6) |
| D765 | IOC2 in 2μ URA3 vector (BamHI-HindIII) | This study |
| D766 | APC9 in 2μ URA3 vector (SacI-XhoI) | This study |
| D767 | HRT3 in 2μ URA3 vector (SacI-XhoI) | This study |
| D768 | ERG27 in 2μ URA3 vector (SpeI-KpnI) | This study |
| D769 | YLR099W in 2μ URA3 vector | This study |
| D770 | ICT1 in 2μ URA3 vector (SacI-XhoI) | This study |
| D619 | KIN1 in 2μ URA3 vector | Benjamin Turk |
| D622 | KIN1-D289A, high-copy-number URA3 vector | Patrick Brennwald |
| D197 | KIN2 in 2μ URA3 vector | Patrick Brennwald |
| D733 | KIN2-D248A, high-copy-number URA3 vector | This study |
| D51 | UPRE-lacZ in high-copy-number URA3 vector | Lee et al. (6) |
| D763 | p59 in high-copy-number URA3 vector | This study |
| D764 | p2 in high-copy-number URA3 vector | This study |
| D762 | p41 in high-copy-number URA3 vector | This study |
| D1008 | HAC1-NRE in low-copy-number URA3 vector | This study |
| D995 | ND-GFP2 in low-copy-number LEU2 | This study |
| D102 | STE2-YFP in high-copy-number TRP vector | This study |
| D1006 | IRE1-YFP in high-copy-number LEU2 vector | Mannan et al. (21) |
| D625 | KIN2-GFP in low-copy-number URA3 vector | Benjamin Turk |
| D1129 | KIN2KD in pEMBLyex4 | This study |
| D1130 | HAC1-NRE-ΔBE in low-copy-number URA3 vector | This study |
| D1131 | HAC1-NRE-GG1143-1144CC in low-copy-number URA3 vector | This study |
a
Affiliations: Thomas E. Dever, National Institutes of Health, Bethesda, MD; Benjamin E. Turk, Yale University School of Medicine, New Haven, CT; Patrick Brennwald, University of North Carolina, Chapel Hill.