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Verification of pRB methylation with peptide substrates. Spectra are derived from MALDI-TOF mass spectrometry analysis of 15-mer synthetic peptides harboring the R798 (a), R775 (b), and R787 (c) residues after in vitro methylation reactions with the peptide alone, the peptide plus PRMT4 (WT), or the peptide plus PRMT4 (EQ).
