FIG 4.

Wild-type RASSF1A and the A(133)S SNP variant of RASSF1A differentially modulate DNA repair. (A) NCI-H1299 cells were stably transfected with vectors expressing wild-type RASSF1A or the A(133)S SNP variant form of RASSF1A. Expression of the exogenous protein was measured by immunoblotting with anti-RASSF1A antibodies and found to be within 5-fold of that observed for the endogenous protein in Calu-6 cells. (B) The cells were irradiated with UV and assayed for changes in DNA repair activity by Comet assay. Box-and-whisker plots show data from 100 cells from two independent experiments. Values that are significantly different are indicated by asterisks as follows: *, P < 0.001 compared to the value for untreated control cells; **, P < 0.01 compared to the value for UV-treated control cells; ***, P < 0.0001 compared to the value for UV-treated RASSF1A-expressing cells. (C) Representative comet tails after irradiation.