FIG 7.

Sulfatide modulates the conformation of PLP at the plasma membrane. Surface expression of PLP-eGFP in differentiated OLN-PLP, OLN-PLP-G, and OLN-PLP-GS cells with three different anti-PLP antibodies against extracellular epitopes, i.e., the first (4C2) and second (O10, ET3) extracellular loops. Note that O10 recognizes surface PLP-eGFP only when sulfatide is present (A). Quantification of the number of O10-positive cells among the total number of cells is shown in panel B. Each bar represents the mean value plus the SD. Statistically significant differences from the results obtained with OLN-PLP cells were assessed by one-way ANOVA with Tukey's posttest (n = 3; ***, P < 0.001). The scale bar is 10 μm.