FIG 3.

FHL2 interacts with LXRs. (A and B) HA-tagged FHL2 and LXRα (A) and FLAG-tagged LXRβ (B) were expressed in HeLa cells, as indicated. Whole-cell extracts were immunoprecipitated, and the bound protein was analyzed by Western blotting with the indicated antibodies. Data are representative of at least three independent experiments. (C) Whole-cell extracts from WT and FHL2-KO SMCs were prepared and immunoprecipitated with anti-FHL2 antibody and analyzed by Western blotting with anti-LXRα antibody. (D) Schematic representation of various truncated mutants of FHL2. (E and F) Cells were cotransfected with expression vectors encoding LXRα (C) or LXRβ (D) and FHL2 mutants, as indicated. Whole-cell extracts were prepared and immunoprecipitated (IP) with anti-HA or Myc antibody. Input samples and immunoprecipitated samples were resolved by 12% SDS-PAGE and analyzed by Western blotting with the indicated antibodies. Data are representative of at least three independent experiments.