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. 2014 Dec 24;7:172. doi: 10.1186/s13068-014-0172-0

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© Comba et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Engineering E. coli for TAG biosynthesis. Heterologous proteins expressed in E. coli are highlighted in color, and the knockout of the E. coli DgkA is labeled with a red cross. Sco0958, Lppβ, and ACC correspond to diacylglycerol acyltransferase, phosphatidic acid phosphatase, and acetyl-CoA carboxylase enzymes from S. coelicolor, respectively. (1) Glycolytic pathway; (2) acetyl-CoA carboxylase; (3) malonyl-CoA:ACP transacylase; (4) glycerol-3-P dehydrogenase; (5) glycerol-3-P and lysophosphatidic acid acyltransferases; DHAP, dihydroxyacetone phosphate; FAS, fatty acid synthase; TCA, tricarboxylic acid; MDO, membrane-derived oligosaccharide.