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. 2014 Dec 24;7:172. doi: 10.1186/s13068-014-0172-0

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© Comba et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TLC analysis of total lipid extracts from E. coli MPS11 derivative strains. Cultures of E. coli were grown as described in Methods, and expression of Sco0958 and Lppβ enzymes was induced with IPTG and L-arabinose, respectively. pBAD33 was used as an empty plasmid control. Lipid pattern obtained after processing 1 mg cell dry mass visualized by Cu-phosphoric staining. L-Ara, L-arabinose; TAG, triacylglycerol; FA, fatty acids; DAG, diacylglycerol.