Table 1.
Strains and plasmids
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| DH5α | E. coli K12 F - lacU169 (Φ80lacZ∆M15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1 | [40] |
| BL21 (DE3) | E. coli F - ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3) | Novagen |
| MPS10 | BL21 (DE3) ∆dgkA::Km; KmR | This study |
| MPS11 | BL21 (DE3) ∆dgkA | This study |
| MPS12 | BL21 (DE3) ∆dgkA ∆fadE::Km; KmR | This study |
| MPS13 | BL21 (DE3) ∆dgkA ∆fadE | This study |
| MPS14 | BL21 (DE3) ∆dgkA ∆fadE ∆araBAD::Km; KmR | This study |
| MPS15 | BL21 (DE3) ∆dgkA ∆fadE ∆araBAD | This study |
| Plasmids | ||
| pET28a | Vector for expression of N-terminal His-tagged proteins under the strong T7 promoter; KmR | Novagen |
| pBAD33 | Vector for recombinant protein expression under the control of the P BAD promoter; CmR | [41] |
| pKD13 | Template plasmid for amplification of the FRT-flanked kanamycin cassette; ApR KmR | [39] |
| pKD46 | Temperature-sensitive replication plasmid for Red recombinase expression; ApR | [39] |
| pCP20 | Temperature-sensitive replication plasmid for thermal induction of FLP synthesis; CmR ApR | [42] |
| pCR®-BluntII-TOPO | Vector used for cloning of blunt PCR products; KmR | Invitrogen |
| pBAD0958 | pBAD33 carrying the SCO0958 His gene under the control of P BAD promoter; CmR | [15] |
| pET28-LPPβ | pET28 carrying the SCO1753 His gene under the control of T7 promoter; KmR | [12] |
| pBAD-LPPβ | pBAD33 carrying the SCO1753 His gene under the control of P BAD promoter; CmR | [12] |
| pTR257 | pET28 carrying the SCO0958 His gene under the control of T7 promoter; KmR | [15] |
| pET28-0958- LPPβ | pET28 carrying the SCO0958 His-SCO1753 His operon under the control of T7 promoter; KmR | This study |
| pBAD-0958- LPPβ | pBAD33 carrying the SCO0958 His-SCO1753 His operon under the control of P BAD promoter; CmR | This study |
| pET28-AtfA | pET28 carrying the atfA His gene under the control of T7 promoter; KmR | This study |
| pET28-FadD | pET28 carrying the fadD His gene under the control of T7 promoter; KmR | This study |
| pET28-0958- FadD | pET28 carrying the SCO0958 His-fadD His operon under the control of T7 promoter; KmR | This study |
| pCC01 | pET28 carrying the accA2 His gene under the control of T7 promoter; KmR | [43] |
| pET28-AccA2-AccE | pET28 carrying the accA2 His-accE His operon under the control of T7 promoter; KmR | This study |
| pET28-AccA2-AccE-AccB | pET28 carrying the accA2 His-accE His-accB His operon under the control of T7 promoter; KmR | This study |
| pET28-0958-ACC | pET28 carrying the SCO0958 His-accA2 His-accE His-accB His operon under the control of T7 promoter; KmR | This study |
| pET28-GpsA | pET28 carrying the gpsA His gene under the control of T7 promoter; KmR | This study |
| pET28-0958-GpsA | pET28 carrying the SCO0958 His-gpsA His operon under the control of T7 promoter; KmR | This study |
| pET28-FadR | pET28 carrying the fadR His gene under the control of T7 promoter; KmR | This study |
| pET28-0958-FadR | pET28 carrying the SCO0958 His-fadR His operon under the control of T7 promoter; KmR | This study |
| pET28-0958-0958 | pET28 carrying the SCO0958 His-SCO0958 His operon under the control of T7 promoter; KmR | This study |