Table 2.
PCR oligonucleotides
| Name | Sequence (5′-3′) | Reference |
|---|---|---|
| dgkA_F | AGGATCTGCCGGAATAGACTTGCTT | This study |
| dgkA_R | AGCGCCTTCAGATGTTCTTCAGCC | This study |
| fadE_F | CAAAAGCGAGAAGTACGGGCAGGTG | This study |
| fadE_R | GCTTTCGATTGATGGTAAAACGGTG | This study |
| araBAD_F | CTCGATTTTGGCAGTGATTCTGTGCGAGCTTTGGCGGTGGCTGTCAAACATGAGAATTAA | This study |
| araBAD _R | CCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTGTGTAGGCTGGAGCTGCTTC | This study |
| ara_check_F | TAAACGAGTATCCCGGCAGCAGGGG | This study |
| ara_check _R | CGGGAATAAACGCCACGGACTCTTG | This study |
| araA_wt_F | AAGTGTATTACGGGTTTCGTCGCTA | This study |
| atfA_F | CATATGCGCCCATTACATCCGATTG | This study |
| atfA_R | ACTAGTCCTTTAGTTTTATCTGATA | This study |
| fadD_F | GCTAGCAAGAAGGTTTGGCTTAACC | This study |
| fadD_R | ACTAGTCTCAGGCTTTATTGTCCAC | This study |
| gpsA_F | CATATGAGCAAGCCGGTCAAGGCGG | This study |
| gpsA_R | ACTAGTGCGGGTTGCGCGGGGGGTC | This study |
| fadR_F | CATATGGTCATTAAGGCGCAAAGCC | This study |
| fadR_R | ACTAGTATGGGAAATCTGTAAAAAC | This study |
| accB_F | CATATGACCGTTTTGGATGAGGCGC | This study |
| accB_R | ACTAGTTCACTGCGGCGGGTTGCCG | This study |
| accE_F | CATATGTCCCCTGCCGACATCCGCG | This study |
| accE_R | ACTAGTTCAGCGCCAGCTGTGCGGG | This study |
Restriction sites are shown in italics. Homologous sequences to pKD13 plasmid are shown in bold.