Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Oligonucleotide primers used for site-directed mutagenesis.
| Mutant | Orientation | Primer sequencea |
|---|---|---|
| NS4A V67I | Forward | GCCTTATTGAGTGTGATGACCATGGGAATAT TCTTCCTCCTCATGCAGCGGAAGGGC |
| Reverse | GCCCTTCCGCTGCATGAGGAGGAAGAATAT TCCCATGGTCATCACACTCAATAAGGC |
|
| NS4B I240M | Forward | GGGGGTTGGTTGTCATGTCTATCCATGACA TGGACACTCATAAAGAACATGGAAAAACC |
| Reverse | GGTTTTTCCATGTTCTTTATGAGTGTCCATGT CATGGATAGACATGACAACCAACCCCC |
aMutated positions relative to NY99 isolate (KC407666) are shown in bold.
