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. 2015 Jan 15;5:772. doi: 10.3389/fmicb.2014.00772

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Copyright © 2015 Marx, Meiers and Brückner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Change of CiaR-dependent promoter activities upon acetate supplementation in strains harboring varriants of ciaH. The strains were grown in BHI medium containing 50 mM of sodium acetate or BHI without the addition of acetate to an OD₆₀₀ of 0.4 and ß-galactosidase was measured. Activities of two promoters, P_htrA and P_spr0931, were determined under both growth conditions and the relative expression rate was calculated by dividing the value of the acetate supplemented culture by the value of the untreated sample. All strains contained promoter fusions in the genome (bgaA::tetM-P_spr0931-lacZ or bgaA::tetM-P_htrA-lacZ). The following strains were used (from left to right): R6, RKL168 (ciaH306), RKL162 (ciaH202), RKL163 (ciaH305), RKL164 (ciaH208), RKL165 (ciaH408), RKL243 (ciaH232), RKL245 (ciaH556), RKL246 (ciaH1057), RKL244 (ciaH_TpVT). The ciaH allele numbers are indicated. Values of at least two independent cultures of each promoter harboring strain are shown along with standard deviations.