Table 4.

Amplification specificity of TMPCR and 16S rDNA-specific PCR.

Organism	Strains tested   (n = 100)	Specificity (%)
		TMPCRa	16S rDNA PCRb
V. cholerae O1	21	100	100
V. cholerae O139	16	100	100
V. cholerae NAG Ic	16	100	100
V. cholerae NAG IId	16	100	100
Other vibrios	8	0	100
Gram-negative bacteria	16	0	100
Gram-positive bacteria	7	0	100

^aThe specificity (%) = strains showing a positive amplification of expected amplicon sizes by TMPCR as having no cross-hybridization with negative controls/a total of strains tested, multiplied by 100.

^bAs for 16S rDNA-specific PCR, no cross-hybridization occurred in reactions containing nuclease-free water or no DNA.

^cNAG I non-O1/non-O139 growing in semisolid agar in the presence of sucrose and mannose but not arabinose.

^dNAG II non-O1/non-O139 growing in semisolid agar only supplemented with sucrose.