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. 2013 Jan 18;1(1):69–81. doi: 10.18632/oncoscience.10

Figure 1. Experimental design for in vitro isolation of RNA from migratory/invasive and reference populations.

Figure 1

A. After 24h of serum starvation, MDA-MB-231 cells were seeded into Transwell inserts with (invasion) or without a layer of Matrigel (migration). At the indicated early and late time points, total RNA was isolated after direct lysis of the respective cell populations on the membranes. This procedure was performed in triplicate for each condition. B. Time point selection for RNA-isolation from migratory and invasive cells. Dynamic migration (left) and invasion (right) profiles of MDA-MB-231 cells have been generated by xCELLigence RTCA in correlating conditions with the Transwell experiments (panel A). Arrowheads indicate selected time points for RNA-isolation as described in panel A. Inset shows an integrated plot of the migration (blue graph) and invasion (red graph) patterns.