Table 1. Number and percentage of TKI resistant clones recovered from ENU-treated cells with or without MS-5 feeder and after 5-6 week culture in 96-well plates.
| Exp. | TKI | UT-7 cell line | Without MS-5 feeder | With MS-5 feeder |
|---|---|---|---|---|
| 1 | imatinib | UT-7-11 | 54 (56%) | 51 (53%) |
| 2 | nilotinib | UT-7-11 | 31 (32,3%) | 0 (0%)* |
| 3 | dasatinib | UT-7-11 | 12 (12.5%) | 11 (11.5%) |
| 4a | ponatinib | UT-7-315 | 65 (68%) | 96 (100%) |
| 4b | ponatinib | UT-7-315 | 68 (71%) | 96 (100%) |
| 5 | ponatinib | UT-7-11 | 0 (0%) | 96 (100%) |
*
No outgrowth observed (viable UT-7-11 located within the MS-5 feeder), 24 wells were replated in methylcellulose in the presence of nilotinib and resistant colonies were observed after 14 days of culture.