Table 2. Resistant clones harboring a BCR-ABL1-KD mutation.

Exp.	TKI	UT-7 cell line	MS-5 feeder	Clones tested for mutation		Results of mutation analysis
				Number	Available**	No mutation	one mutation	two mutations
1	imatinib	UT-7-11	−	24	22	2	20	0
			+	24	23	0	23	0
2	nilotinib	UT-7-11	−	24	24	11	12	1
			+	24*	24	13	11	0
3	dasatinib	UT-7-11	−	12	12	1	11	0
			+	11	11	1	10	0
4	ponatinib	UT-7-315	−	48	47	0	10***	37****
			+	48	46	0	34***	12****
5	ponatinib	UT-7-11	−	0	0	0	0	0
			+	24	24	0	22***	2****

^*Methylcellulose colonies after 14 days of culture in the presence of nilotinib.

^**Clones correctly expanded in 24-well plates.

^***T315I mutation alone.

^****one mutation in addition to the T315I mutation.