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. 2013 Dec 11;1(1):21–29. doi: 10.18632/oncoscience.5

Fig 5. Subcellular localization of heat shock proteins in response to disulfiram/copper.

Fig 5

OVCAR3 cells grown on glass cover slips were treated for 6 h with 1 μM disulfiram/copper, fixed with methanol, and incubated with antibodies against HSP70 (monoclonal), HSP27 (monoclonal), and p65/RelA (polyclonal), followed by an Alexa Fluor 488-coupled secondary antibody (Dianova, Hamburg, Germany). Cells were embedded in mounting medium (Vectashield mounting medium with DAPI, Axxora, Lörrach, Germany) and visualized by phase contrast microscopy and immunofluorescence microscopy using a Zeiss Axiophot fluorescence microscope (Zeiss, Jena, Germany). A) 40x lens, B) digitally zoomed segments of microphotographs showing disulfiram/copper-treated OVCAR3 cells.