Figure 3. Effect of inhibition of laccase2 expression after injection with dsRNA in second-instar larvae of Cylas puncticollis.

(A) Mortality after injection with dsRNA targeting laccase2 (dslac2) (day 14–20) expressed in percentage. Mortality in larvae injected with dsRNA targeting gfp (dsgfp) (control) was only 8% (B) Larvae injected with dsgfp as a control and (C) treated larvae after 3 days; (D) Pupa development 6 days after injection with dsgfp as a control and (E) dslac2; (F) Adult development injected 10 days after injection with dsgfp as a control (G) dslac2 (H) Surviving individual 13 days after injection with dslac2. Larvae were injected with the dsRNA solution into the hemocoel at a concentration of 0.2 µg/mg body weight. The insects were kept in sweetpotato roots after injection for the duration of the experiment.