Table 1. Overview of identified genes related to the RNAi pathways in C. puncticollis.

	Contig	First hit BLASTp	Tribolium homologue	Comparison to Tribolium
miRNA
Dcr-1	Cp.comp36004_c0_seq2	hypothetical protein YQE_09128, partial [Dendroctonus ponderosae]	EFA11550	E = 0.0; bits = 1971
Ago1	Cp.comp34373_c0_seq6	argonaute1 [Tribolium castaneum]	EFA09197	E = 0.0; bits = 1765
Loquacious	Cp.comp35585_c0_seq1	PREDICTED: similar to tar RNA binding protein; [Tribolium castaneum]	XP_966668	E = 0.0; bits = 545
Drosha	Cp.comp39990_c0_seq1	PREDICTED: similar to ribonuclease III [Tribolium castaneum]	XP_967454	E = 0.0; bits = 1684
Pasha	Cp.comp38940_c0_seq1	hypothetical protein YQE_10523, partial; [Dendroctonus ponderosae]	XP_971282	E = 0.0; bits = 786
Exportin-5	Cp.comp39084_c0_seq1	hypothetical protein YQE_01298, partial [Dendroctonus ponderosae]	XP_974696	E = 0.0; bits = 1316
siRNA
Dcr2	Cp.comp37119_c0_seq1	hypothetical protein D910_09530, partial [Dendroctonus ponderosae]	NP_001107840	E = 0.0; bits = 1012
Ago-2	Cp.comp38067_c0_seq1	hypothetical protein D910_08685 [Dendroctonus ponderosae]	EFA04626	E = 0.0; bits = 988
R2D2	Cp.comp37256_c0_seq4	hypothetical protein YQE_06343, partial [Dendroctonus ponderosae]	NP_001128425	E = 1e-83; bits = 266
piRNA
AGO3	Cp.comp32215_c0_seq1	hypothetical protein YQE_10018, partial [Dendroctonus ponderosae]	EFA02921	E = 0.0; bits = 1003
PIWI	Cp.comp31984_c0_seq1	piwi [Tribolium castaneum]	EFA07425	E = 0.0; bits = 989
Aubergine	Cp.comp31984_c0_seq1	piwi [Tribolium castaneum]	XP_001811159	E = 0.0; bits = 975
Zucchini	Cp.comp38142_c1_seq5	hypothetical protein TcasGA2_TC010319 [Tribolium castaneum]	EFA13216	E = 1e-46; bits = 166
Zucchini	Cp.comp38489_c0_seq8	hypothetical protein YQE_07414, partial [Dendroctonus ponderosae]	EEZ99465	E = 1e-50; bits = 176

(FS) frame shift; (RF) reading frame.