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. 2015 Jan 15;9(1):e0003434. doi: 10.1371/journal.pntd.0003434

Figure 5. Expression of fibrosis-related factors in ICOSL KO and WT mice.

Figure 5

(A). The areas of egg granulomas or fibrosis were chosen, and a two-step immunohistochemical method (peroxidase-conjugated polymer) was used on paraffin section of liver as described in the Materials and Methods. The dark brown area represents positive particles and arrow shows eggs of S. japonicum (original magnification×400). (B). The percentage of positive staining cells in 15 granulomas was calculated to assess the expression of IL-13, TGF-β1, MMP-9 and TIMP-1 on inflammatory cells of the granulomas in ICOSL KO mice at 12 weeks (IL-13 and TGF-β1) or 16 weeks (MMP-9 and TIMP-1) post-infection. Each bar represents mean of % positively staining cells in 15 granulomas +/− SD of 5 mice per group from two independent experiments.*P<0.05 between ICOSL KO and WT mice groups, Student’s t-test.