Figure 5. Expression of major Ca²⁺-handling proteins in muscles of WT and Actn3 KO mice.

Protein expression analysis of the EDL (A), FDB (B) and quadriceps (C) muscles of WT and Actn3 KO mice. Representative Western blots are shown with densitometry values normalised to total protein and the average of all WT samples. A significant increase in SERCA1 was observed in the EDL, FDB and quadriceps, along with an increase in calsequestrin (CSQ1) in the EDL and sarcalumenin (SAR) in the FDB and quadriceps. No change in RyR1 and DHPR expression were seen in the EDL and parvalbumin (PARVALB) is unchanged in both the EDL and FDB. A total of 6 WT and 6 Actn3 KO EDL, 3 WT and 3 Actn3 KO FDB and 4 WT and 4 Actn3 KO quadriceps muscles were analysed. Statistical analyses were performed using the Mann-Whitney U non-parametric test (* denotes p < 0.05).