(A) TIR1-myc, AFB4-myc, and AFB5-myc were immunoprecipitated with anti-myc antibody coupled to agarose beads, and ASK1 was detected with an anti-ASK1 antibody. The lower band corresponds to ASK1 protein.
(B) Pull-down reactions were incubated for 45 min in the presence or absence of 50 μM indole-3-acetic acid (IAA). GST-IAA3 was immunoprecipitated with glutathione agarose beads, and AFB4-myc and AFB5-myc protein were detected with anti-c-myc-peroxidase antibody.