Fig. 5.

Effects of MDP (3 mg/kg), FK565 (0.003 mg/kg) and LPS (0.1 mg/kg) on circulating cytokine levels 3 h (A–D) and 26 h (E–H) after treatment of male mice. Mice were injected i.p. with saline (VEH), MDP, FK565, LPS, MDP + LPS or FK565 + LPS. Plasma was collected 3 h (A–D) or 26 h (E–H) later. Circulating levels of IFN-γ (A + E), IL-1β (B + F), IL-6 (C + G), and TNF-α (D + H) were measured. Note that the scale of the ordinate is different between the 2 time points. The values are means + SEM, n = 7–8. (A–D): Post-hoc analysis of significant NOD × LPS interactions in 2-way ANOVA: ^∗p < 0.05, versus VEH. ^#p < 0.05, ^##p < 0.01, MDP + LPS versus MDP or FK565 + LPS versus FK565. ^§p < 0.05, ^§§p < 0.01, MDP + LPS or FK565 + LPS versus LPS. ^Φp < 0.05, ^ΦΦp < 0.01, MDP + LPS versus FK565 + LPS. (E–H): One-way ANOVA: ^∗p < 0.05, ^∗∗p < 0.01, versus VEH.