Fig. 6.

Effects of MDP (3 mg/kg), FK565 (0.003 mg/kg) and LPS (0.1 mg/kg) on cytokine mRNA expression in the brain 3 h (A–D) and 26 h (E–H) after injection in male mice. Mice were injected i.p. with saline (VEH), MDP, FK565, LPS, MDP + LPS or FK565 + LPS. Expression of IFN-γ (A + E), IL-1β (B + F), IL-6 (C + G), and TNF-α (D + H) was measured 3 h (A–D) or 26 h (E–H) after injection. The values are means + SEM, n = 7–8. (A–D): Post-hoc analysis of significant NOD × LPS interactions in 2-way ANOVA: ^#p < 0.05, ^##p < 0.01, MDP + LPS versus MDP or FK565 + LPS versus FK565. ^§p < 0.05, ^§§p < 0.01, MDP + LPS and FK565 + LPS versus LPS. Main factor effects without NOD × LPS interactions: ^ap < 0.01, LPS versus VEH. ^bp < 0.05, ^bbp < 0.01, NLR agonists versus VEH. (E–H): One-way ANOVA: ⁺p < 0.1, ^∗p < 0.05, ^∗∗p < 0.01, versus VEH or as indicated by the brackets.