Fig. 8.

Effects of MDP (3 mg/kg), LPS (0.83 mg/kg) and MDP + LPS on c-Fos expression 3 h after treatment of male mice. Mice were injected i.p. with saline (VEH), MDP, LPS or MDP + LPS. Brains were collected 3 h post-treatment and the expression of c-Fos, a marker of neuronal activation, was measured by immunohistochemistry. The values are means + SEM, n = 3–5. Post-hoc analysis of significant NOD × LPS interactions in 2-way ANOVA: ^∗p < 0.05, ^∗∗p < 0.01, versus VEH. ^##p < 0.01, MDP + LPS versus MDP. ^§p < 0.05, ^§§p < 0.01, MDP + LPS versus LPS. Main factor effects without NOD × LPS interactions: ^ap < 0.01, LPS versus VEH. ^bp < 0.01, NLR agonist versus VEH. Abbreviations: BNSTd/v = bed nucleus of the stria terminalis dorsal/ventral, CeA = central amygdala, DG = dentate gyrus, PVN = paraventricular nucleus of the hypothalamus, SFO = subfornical organ, SO = supraoptic nucleus.