. 2014 Oct 9;125(3):465–469. doi: 10.1182/blood-2014-07-588954

Table 1.

Intraaortic hematopoietic clusters contain pre-HSCs

		Cell number	Neonate recipients^§
Embryo stage	Sorted cells	injected/recipient	Rag2^−/−γc^−/−	WT
Early E10 (25-32 sp)	c-kit⁺^*	10	0/1	0/9
		50	—	0/4
		100	2/2	0/2
		150	—	—
		200	—	3‖/5
		750	1^¶/1	—
		1350	1/1	—
	c-kit⁺GFP^–CD45^–^†^‡	1000-2500	3/3	—
	c-kit⁺GFP⁺CD45^–	250-550	3/4	—
	c-kit⁺GFP^–CD45⁺	150-350	1/4	—
	c-kit⁺GFP⁺CD45⁺	250-650	3/4	—
Mid-E10 (33-38 sp)	c-kit⁺^*	10	0/1	0/6
		50	2/2	2^¶^#/12
		100	—	1/3
		150	2/2	—
		200	—	1/1

—, Not done.

n = 5 independent experiments.

^†

n = 3 independent experiments.

^‡

The doses of c-Kit⁺GFP^–CD45^–, c-kit⁺GFP⁺CD45^–, c-kit⁺GFP^–CD45⁺, and c-kit⁺GFP⁺CD45⁺ cells injected per recipient correspond to 5 to 12, 5 to 11, 4 to 9, and 5 to 13 ee injected per recipient, respectively.

^§

Number of repopulated recipients per injected neonate recipients 4 to 5 months postinjection. Mice were considered repopulated when donor-derived cells (>0.01%) were detected in both the bone marrow and spleen by flow cytometry of the H2k^k and Ly6a-GFP donor markers (for Rag2^−/−γc^−/− recipients) or of the CD45.1 donor marker (for WT recipients).

‖Multilineage reconstitution is shown in supplemental Figure 5.

^¶

Primary recipients used for secondary transplantations (see supplemental Table 1).

Multilineage reconstitution is shown in Figure 1H.