Fig. 1.

Expression of TRPV1 in salivary gland epithelial cells (SGEC). (A) Expression of TRPV1 mRNA in mouse SMG (mSMG), human SMG (hSMG), and HSG cells by RT-PCR, with GAPDH as an internal control. (B) Expression of TRPV1 protein in mSMG, hSMG, and HSG cells by Western blot. Approximately 50 µg of protein was separated on 8% SDS-polyacrylamide gel electrophoresis and TRPV1-immunoreactivity was determined using a polyclonal anti-TRPV1 antibody. The blot is representative of five separate experiments with similar results. (C) Quantitative analysis of TRPV1 between DRG and SMG in mice by real-time PCR. TRPV1 mRNA levels are represented as ratios to the mRNA level of the housekeeping gene, GAPDH. Results are presented as mean±SD (n=4, ^**p<.001).