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. 2014 Oct 24;104(3):501–511. doi: 10.1093/cvr/cvu231

Figure 5.

Figure 5

Reconstitution of wt and mutant IKs in adult rat ventricular cardiomyocytes. (A) Exemplar current traces measured in the absence (traces a) and presence (traces b) of 30 µM chromanol 293B in a cardiomyocyte expressing wt KCNQ1 + KCNE1. The difference traces (a–b) represent the chromanol 293B-sensitive currents, i.e. IKs. (B) Population I–V curves for chromanol 293B-sensitive currents in adult rat cardiomyocytes expressing wt KCNQ1 + KCNE1, n = 26. (C and D) Data for cardiomyocytes expressing KCNE1 alone indicate the lack of IKs (n = 4), same format as A and B. (E–J) Data obtained from cardiomyocytes expressing R555H + KCNE1 (n = 21), G589D + KCNE1 (n = 10), and L619M + KCNE1 (n = 16), respectively, same format as A and B. Data for wt KCNQ1 + KCNE1 channels are reproduced (red trace) in E–J for visual comparison. Data points are mean ± SEM. Data were generated from three different rat cardiomyocyte preparations. Scale bars, 5 pA/pF, 1 s.